Other

Part:BBa_K4877016:Design

Designed by: Team iGEM Bielefeld-CeBiTec 2023   Group: iGEM23_Bielefeld-CeBiTec   (2023-10-12)


DART VADAR sensor cloning site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal XhoI site found at 44
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An illegal PstI restriction site in the left homology arm had to be mutated.


Source

The sequence was taken from the published pDV06 plasmid by Gayet et al. [1].

[1] Gayet, R.V., Ilia, K., Razavi, S. et al. Autocatalytic base editing for RNA-responsive translational control. Nat Commun 14, 1339 (2023). https://doi.org/10.1038/s41467-023-36851-z

References